Bovine blood agar with esculin
The figure shows plates with Bovine Blood agar with Esculin, and no bacteria have been cultivated on plate A. On plate B - Streptococcus agalactaie, has been cultivated. Note that Streptococcus agalactaie is incapable of esculin hydroysis, appear with a strong beta-hemolysis with no darkening of the media, while Streptococcus uberis on playte B is non-haemolytic with darkening of the media. The plate is shown with lighting from above (A) and from below (B). - Click on the image to enlarge it.
Image: Lise-Lotte Fernström (HBIO, SLU) and Ingrid Hansson (HBIO, SLU). - Click on the image to enlarge it. ApplicationsBovine blood agar with esculin is an agar medium for all common udder pathogenic bacteria. Bovine blood agar with esculin is mainly used in analysis of mastitis samples as it can distinguish ulin to differentiate group D streptococci from Streptococcus agalactae, as S. agalactiae is not capable of esculin-hydrolysis. When esculin is hydrolysed by organisms it forms dextrose and esculin, which react with a compound in the media to produce a darkening around the colonies. The medium contains:
The final pH of this medium used to be 7.6
Updated: 2024-11-13. |
