VetBact

Phylum	Class	Order	Family	Genus	Species/Subspecies

Species/Subspecies:

Staphylococcus pseudintermedius

Category:

Causes hemolysis

Etymology:

Genus name: bunch of grape-like cocci (coccus means a grain or berry)
Species epithet: refers to the similarity with S. intermedius.

Significance:

[Very important]

Taxonomy:

Phylum
Bacillota

Class
Bacilli

Order
Bacillales

Family
Staphylococcaceae

Genus
Staphylococcus

Type Strain:

LMG 22219 = CCUG 49543

Macromorphology (smell):

Small round, greyish white, opaque colonies (1-2 mm in diameter) with smoth margin. Does often give a double hemolysis zone on blood agar [clear (complete) narrow inner zone and a broad turbid (patial) outer zone].

The complete hemolysis of Staphylococcus spp. is caused by an α-hemolysin and the partial hemolysis by a β-hemolysin. Cf. Streptococcus spp.

Micromorphology:

Cocci (0.5-1.5 µm in diameter), arranged in groups

Gram +/Gram -:

G+

Metabolism:

Facultatively anaerobic

Catalase/Oxidase:

+/-

Other Enzymes:

Coagulase +, DNase +, esculinase - and urease +

Biochemical Tests:

Voges-Proskauer +

Fermentation of carbohydrates:

D-glucose
+

lactose
+

maltose
+

L-rhamnose
-

sucrose
+

L-arabinose
-

cellobiose
-

D-mannitol
w

salicin
-

trehalose
+

glycerol
w

inulin
-

raffinose
-

D-sorbitol
-

starch
-

Comments: Maltose is, however, negative or possible weakly positive under aerobic conditions on agar plates (see Fig. 135:5).
Other carbohydrates: D-xylose -.

Spec. Char.:

Special Media:

S. pseudintermedius is a lactose fermenter, which can easily be seen on both purple agar och CLED agar.

Disease:

Hosts	Disease	Clinical picture
Dog, cat	Dermatitis, otitis, woundinfection and mastitis	Opportunist, included in the normal skin flora of dogs. The most common cause of skin and wound infection in dogs, less common in cats
Dog, cat	Urinary tract infections and endometritis	Poluri and polydipsi
Other animal species incl humans	Skin and wound infections	Relatively uncommon in animal species other than dogs and cats

Genome Sequence:

Acc-no	Strain	Size (bp)	Genome
CP002478	ED99	2 572 216	1c + 1c

At least two strains have been sequenced. Plasmids have not been reported.

16S rRNA Seq.:

Acc-no	Strain	Number of NT	Operon	Sequence similarity
AJ780976	(T)	1512	5	100%

Taxonomy/phylogeny:

About 70 species and subspecies have been described within the genus Staphylococcus. S. pseudintermedius is closely related to S. intermedius, S. delphini and S. schleiferi.

Comment:

S. pseudintermedius is coagulase-positive and can, therefore, be confused with primarely S. intermedius, but also with S. aureus. Purple agar with maltose is used for differentiation of S. aureus and S. pseudintermedius (see above).

Reference(s):

No. 56

Updated:

2023-03-08

VetBact

Recently Updated

Recent blog posts