Cefsulodin-Irgasan-Novobiocin (CIN) agarApplications:CIN (Cefsulodin-Irgasan-Novobiocin) agar is a differential and selective medium used in qualitative procedures for the isolation of Yersinia enterocolitica from different clinical and nonclinical samples. Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enterica, Shigella sonnei and Streptococcus faecalis do not grow on CIN agar. By reducing the concentration of cefsulodin, CIN Agar could also be used for selective isolation of Aeromonas spp. The medium contains:
The final pH of this medium should be 7.4. Other comments:Differentiation of bacteria is based on mannitol fermentation. Organisms which are capable of fermenting mannitol will decrease the pH around the colony and the corresponding colour change of neutral red will generate colonis that appear as red. Because of the localized pH decrease, a zone of precipitated bile may also be present. The appearance of the colonies has some similarity with a "bull's eys". Organisms that do not metabolize mannitol to acid will form colorless, translucent colonies. Updated: 2018-05-09. |