VetBact

Applications:

"Tryptic soy agar (TSA)" is a general medium, which is used for isolation and enumeration of many different bacteria, among others, E. coli.

The medium contains:

• casein peptone (contains amino acids and peptides)
• soya peptone (contains amino acids and peptides)
• NaCl (contributes to the osmotic balance)
• agar (gelifying agent)
• water

The final pH of the medium should be 7.3.

Other comments:

TSA is often used in combination with VRB agar. TSA is then mixed with the sample to be analyzed at around 40°C and poured into the plates. After preincubation for 1-2 h at ambient temperature, the TSA is overlayed with VRB agar and the incubation is then continued at 37°C.

Updated: 2018-05-09.